2.2.6. Water holding capacity (WHC)
WHC was determined using the method outlined by Zarai, Balti,
Mejdoub, Gargouri, and Sayari (2012) with slight modification. One
gram of the samples was weighed into a pre-weighed 50 mL
centrifuge tube and mixed with 50 mL of distilled water. The
samples were then allowed to stand at 30 ± 2 C for 1 h before
centrifuging at 450 g for 20 min. After centrifugation, the supernatantwas
decanted and centrifuge tubewas drained for 30 min on
a filter paper after tilting to a 45 angle and weighed. The WHC was
expressed as grams of water held by 100 g of protein.
2.2.7. Fat binding capacity (FBC)
The above method was used for measuring the FBC, but instead
of 50 mL distilled water, 10mL of corn oil was added to the samples.
The FBC was expressed as grams of oil held by 100 g of protein