the extraction method have presented high repeatability and high reproducibility. the standard for the percentage of procovered lipid was between 0.3% and 2.3%. regarding the amount of lipids extracted by each method the Bligh and Dyer method and the Folch method have presented the lowest standard error 5%. thus higher repeatability.the producibility of all method was very high. the average values calculated after three run with microalgae produced by different batches resulted in a reproducibility of 97% for the Bligh and Dyer. folch and chen method and of 96% for the soxhlet and the hara and radin method. reproducibility was calculated at the standard error between different batches divided by the average value between different batches.