Analysis using a cryogenic oven with GC is known as a sensitive
technique to detect volatile organic compounds in biological
samples such as blood and urine [8,9]. To our knowledge, the first
report dealing with GC with a cryogenic oven temperature device
in biological samples was the analysis of chloroform or methylene
chloride in whole blood [10]. This device was originally designed
to rapidly cool the oven to reduce analysis time. Watanabe et al.
[10] reported that the method using that device was very sensitive
and showed low background noise. Accordingly, we used this
assay to analyze acetylene. The molecular weight of acetylene is
26.04, which is less than that of nitrogen. Good chromatographic separation from air and low background noise are indispensable to
quantify acetylene.