Aqueous extract of A. sativum was
the only herb extract with a MIC >500 μg/mL. Based on mortalities in 2 weeks after intraperitoneal S. agalactiae injection, the
median lethal dose (LD50) of S. agalactiae for Nile tilapia (Oreochromis niloticus) was 3.79×105 CFU/mL. In vivo experiments
showed that fish feed supplemented with either A. paniculata leaf powder or dried matter of A. paniculata aqueous extract
reduced mortality of S. agalactiae infected Nile tilapia. In addition, no mortality was found in fish receiving dried matter of A.
paniculata aqueous extract supplemented feeds at ratios (w/w) of 4:36 and 5:35.