Mechanism
First of all in this procedure thickness of flagella is increase so it can be visible.
The Leifson’s stain is made up of tannic acid ,basic fuschin stain prepared in alcohol base.
When we treat Leifson’s stain with cell the tannic acid get attach to the flagella and alcohol get evaporated.
After evaporation of alcohol the thickness of flagella is increased due to deposition of tannic acid.
Where as Basic fuschin stain the Flagella.
After Leifson’s stain treatment cells are treated with Methylene blue stain.
This Methylene blue stains the cell.