The faeces samples collected from rectum and feed samples were dried in a forced draft oven at 65°C for at least 48 h till constant weight,
and ground through a 1-mm screen in a mill.
Dry matter (DM) content was assessed by oven drying at 105°C for 8 h,
following the routine procedures given by AOAC (2004).
The faeces and feed samples were analyzed for crude protein (CP),
crude fat (CF),
calcium (Ca),
and phosphate (P)
according to AOAC (2004).
The CP content was calculated
using Kjeldahl method of nitrogen analysis, and the CF content was analyzed using Soxhlet extraction.
Acid insoluble ash (AIA) content was used
as an internal marker to determine the apparent digestibility of the experimental diets as reported
by Van Keulen and Young (1977).
The faeces samples collected from rectum and feed samples were dried in a forced draft oven at 65°C for at least 48 h till constant weight, and ground through a 1-mm screen in a mill. Dry matter (DM) content was assessed by oven drying at 105°C for 8 h, following the routine procedures given by AOAC (2004). The faeces and feed samples were analyzed for crude protein (CP), crude fat (CF), calcium (Ca), and phosphate (P) according to AOAC (2004). The CP content was calculatedusing Kjeldahl method of nitrogen analysis, and the CF content was analyzed using Soxhlet extraction.Acid insoluble ash (AIA) content was usedas an internal marker to determine the apparent digestibility of the experimental diets as reported by Van Keulen and Young (1977).
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