In a food processing environment, different temperatures may be
encountered and time allowed for biofilm formation varies depending
on the interval between cleaning regimes and design of the equipment.
Both parameters are expected to influence growth and biofilm forming
capacity. Therefore, biofilm formation of the seven L. plantarum strains
was tested at four different temperatures (20, 25, 30 and 37 °C) on polystyrene
coupons (Fig. 2) as described in theMaterials andmethods section.
For these experiments, the biofilm was grown in 12 wells plates
which have a larger surface area compared to the 96 wells plates used
in the screening described above, resulting in larger values for the CV
assay.