Measurement of carotenoid contents. 1.0 g sample was cut from pericarp in a 5 mm
wide strip around the equator of B, B17 and B114 of wild type and 35S:FYFL lines,
respectively. Then 10 ml of 60540 (v/v) hexane-acetone was added respectively and
total carotenoids fruits were extracted. The extract was centrifuged at 4000 g for
5 min and the absorbance of supernatant was measured at 450 nm. Carotenoid
content was calculated with the following equations: total carotenoid mg ml21 5
4*(OD450)*10 ml/1 g43,59. Three independent experiments were performed for each
sample.