Growth & Cultural Characteristics Alkaline Peptone Water: pH 8.6 is useful for prelimnary enrichment of vibrios from faeces or other contaminated materials.
Thiosulphate Citrate Bile-salt Sucrose Agar(TCBS) Most widely selective media. Give good growth of V. cholerae parahaemolyticus and other vibrios within 37 °C Colonies are 2-5 mm in diameter.
Colonies of sucrose fermenting vibrios are yellow e.g.,
V. cholerae. Colonies of sucrose non-fermenting vibrios are green e.g.,
V. parahaemolyticus. Inhibits most enterobacteria and gram positive bacteria though allowing some strains of Proteus, Aeromonas, and enterococci to form small colonies (1 mm ).