The International Honey Commission (IHC) collaboratively
tested three methods for the determination of HMF: two are spectrophotometrical
methods (Winkler’s [17] and White’s methods
[18]), whereas the thirdwas a RP-HPLC [19] method. Comparison of
their performance was reported by Zappalà et al. [20]. Spectrophotometric
methods are fast but low on specificity and sensitivity, on
the other hand, the RP-HPLCmethod is more accurate and sensitive
than the spectrophotometric ones, but is quite slow. In addition, the
application of this method to HMF evaluation in strawberry tree
honey samples has recently evidenced a specific and heavy bias
[21].