The composition of medium used for the production of pectin lyase is as follow (g/L): glucose 2; MgSO4·7H2O 0.05; CaCl2·2H2O, 0.1; NH4Cl, 0.12 and thiamine, 0.001 and pH 6.0 with silica gel chips (1.2 mm, dia) in conical flask plugged with cotton wool and sterilized at 15 lbs/in2 pressure (121 °C) for 15 min. Two milliliter of the spores was transferred into growth medium from pre-culture slants. The experiments were performed on orbital shaker by adjusting the speed 100 rpm at 30 °C for 96 h.