An aliquot of the cellular suspension was fixed with three percent formaldehyde
solution for light microscopy and the rest was used for TEM observations.
Light microscopy studies were performed with an Olympus BX50 microscope on
control and treated specimens.
At least 300 control and altered cells were
measured. Photographs were recorded with a digital camera Olympus C-7070
Wide Zoom. They were analyzed using ImageJ software. Three parameters were
measured for each cell: length, width and thickness.