2.2.2. Co-culture fermentations
Four co-culture fermentationswere performedwith: (1) S. cerevisiae
(UFLA YFFBM18.03) and P. acidilactici (UFLA BFFCX 27.1); (2) S. cerevisiae
(UFLA YFFBM 18.03) and L. acidophilus (LACA 4); (3) P. acidilactici (UFLA
BFFCX 27.1) and L. acidophilus (LACA 4); and finally, (4) S. cerevisiae (UFLA
YFFBM18.03), P. acidilactici (UFLA BFFCX 27.1) and L. acidophilus (LACA 4)
strains. An initial population of 7 log CFU/mL of each strain was inoculated
in pasteurized PSM. The suspensionwith inoculated cellswas fractionated
in 25 mL portions, and placed into 50 mL conical tubes. These
tubes were incubated at 37 °C for up to 24 h and afterwards, the
fermented PSMremained for 24 h at±4 °C. The experimentswere performed
in three independent assays. For each repetition two samples
were taken at each time (duplicate).