2.9.2. Assay of DPPH free radical scavenging activity
DPPH free radical scavenging activity was estimated according
to the procedure described by Yi et al. [15] with slight modifications.
Briefly,2.0 mL of sample was mixed with 2.0 mL of 0.2 mM
ethanolic DPPH solution. The reaction mixture was allowed to
stand for 30 min at room temperature in dark.The absorbance at
517 nm was measured with spectrophot ometer. The same proce-
dure was conducte d for control;however,ethanol was used in-
stead of the sample solution.DPPH free radical scavenging
activity was calculated by using the following equation :
DPPH free radical scavenging activity ð%Þ
¼ ðAbsorbance of control
Absorbance of sample =Absorbance of control Þ 100