Cell-free immobilization. Soluble enzymes that are immobilized
to a support structure have excellent flow characteristics
suitable for continuous operations, in contrast to whole-cell
immobilized supports, and offer considerable savings in terms
of capital equipment. GIs from Streptomyces phaeochromogenes
and Lactobacillus brevis were immobilized on DEAEcellulose
(30). The Streptomyces GI immobilized on DEAEcellulose
is being used to produce HFCS in a semicontinuous
plant by the Clinton Corn Processing Company. A GI preparation
from Streptomyces sp. immobilized on porous alumina
exhibited a half-life of 49 days and was found to be suitable for
continuous use in plug-flow reactors.