This study reports for the first time the fractionation of cell homogenate of P. cruentum in aqueous two-phase systems for the development of a process for the potential primary recovery of B-phycoerythrin. It has been shown that tie-line length, volume ratio, molecular weight of PEG and system pH influence the purity of B-phycoerythrin from the top PEG-rich phase. PEG 8000-phosphate ATPS proved to be unsuitable for the primary recovery of B-phycoerythrin since top phase recovery and protein purity resulted in low values (i.e. <56% and 1.4, respectively). The operating conditions established for the PEG 1450-phosphate ATPS extraction resulted in a one-stage process for the potential recovery of B-phycoerythrin from P. cruentum, that concentrated the protein preferentially to the top phase and the contaminants to the opposite phase. Overall, the results reported here demonstrated the potential application of ATPS for the primary recovery of B-phycoerythrin as a first step for the development of a downstream process with commercial application.
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