The products of
RT-PCR amplifi cations were separated on 3% (w/v)
agarose gels and stained with ethidium bromide.
Photographic documentation was performed using a
gel documentation system (GENEFLASH, Syngene
Bio-imaging). For quantitation of relative band
intensities, the pixel intensities of the RT-PCR products
were analyzed using Metamorph software (Molecular
Devices, USA) and normalized relative to the abundance
of the UBQ5 loading control.