Preferably, do not thaw frozen samples before analysis. If frozen sample must be
tempered to obtain analytical portion, thaw suitable portion as rapidly as possible
to minimize increase in number of competing organisms or to reduce potential of
injuring Salmonella organisms. Thaw below 45°C for 15 min with continuous
agitation in thermostatically controlled water bath or thaw within 18 h at 2-5°C.
Aseptically weigh 25 g sample into sterile, wide-mouth, screw-cap jar (500 ml) or
other appropriate container. For nonpowdered samples, add 225 ml sterile lactose
broth.