Conclusion
For the first time, a rapid, simple and highly sensitive LC–MS/MS method was developed and successfully applied to the pharmacokinetic study of AD and PF in healthy volunteers after intravenous infusion administration of HTLPI. The validated method was proven to be precise, accurate and reproducible for the analysis of AD and PF and the results of the pharmacokinetic study would be helpful to provide some references to clinical application of this medicine.