Fluorescent micrograph of acridine orange and propidium iodide double-stained human colorectal cancer cells (HT-29). (A) Untreated cells showed normal structure
without prominent apoptosis. (B) Cells treated with white tea extract after 8 h showed early apoptosis features including membrane blebbing and chromatin condensation.
(C) More cells showed apoptotic features at 16 h which represent intercalated acridine orange amongst the fragmented DNA and nuclear fragmentation. (D) Presence of
reddish-orange colour at 24 h. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)