Acetobacter aceti MTCC 2623 was studied as an alternative microbial source for bacterial cellulose (BC)
production. Effect of media components on cell growth rate, BC production and cellulose characteristics
were studied. FTIR results showed significant variations in cellulose characteristics produced by A. aceti in
different media. Results have shown the role of fermentation time on crystallinity ratio of BC in different
media. Further, effect of six different media components on cell growth and BC production was studied
using fractional factorial design. Citric acid was found to be the most significant media component for
cell growth rate (95% confidence level, R2 = 0.95). However, direct role of these parameters on cellulose
production was not established (p-value > 0.05).
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