White brined Feta cheese was produced from buffalo milk. Four different batches obtained from the same livestock were mixed and used for cheese making. The milk was divided into four replications for each treatment. The general process for the production of Feta cheese from buffalo milk is shown in Fig. 1. The stages that are shown by dashed lines were subject to changes or adjustments in our studies. In three separate experimental setups, the effect of different variables on the cheese making parameters was studied. As will be discussed in the coming sections, the first set of experiments evaluated the influence of the initial acidity of raw full-fat milk. In the second experimental setup the effect of starter culture type, incubation temperature and acidity increase level during incubation was examined. The last experimental setup was designed for the analysis of the effect of homogenization and pasteurization. The measured parameters included the cheese yield, acidity and fat content as well as chemical characteristics of whey.