Fermentation was carried out in 250 mL Erlenmeyer flask at
30 C and 150 rpm for 24 h with 50 mL of enzymatic hydrolysate
adding 6 g/L yeast extract, 10 g/L peptone, 5 g/L (NH4)2SO4, 10 g/L
KH2PO4, 0.5 g/L MgSO47H2O and 0.15 g/L CaCl2H2O. Each flask
was equipped with a needle-pierced silicone stopper to allow
removal of the produced CO2 during fermentation. The initial yeast
inoculum was OD 10 (600 nm) and initial pH was controlled in 4.8.
At the end of fermentation, samples of the broth were centrifuged
at 5000g for 10 min for sugar and ethanol analysis. Reported data is
the average of duplicates.