The wet biomass
(10.1 g obtained from the Walne medium culture or 7.0 g obtained
from the enriched seawater culture) was suspended in
70 mL chloroform/methanol (1:1 v/v) and then sonicated for
15 min at 50 Hz frequency, followed by Soxhlet extraction with
the same solvent at 60 C for 12 h.