A pure colony of test bacteria was taken by using a sterile wire loop and transferred into test tubes having a sterile nutrient broth. The test tubes were incubated at 37 °C for 24 h until the visible turbidity and density equal to that of 0.5 McFarland standard. After adjusting the turbidity, sterile cotton swab was dipped into the suspension and streaked over the entire surface of the plate medium for three times by rotating the plate approximately at 60 °C to ensure uniform distribution of the inoculum