parenchymal cells of experimental sections. These
deposits had characteristic and unvarying locations
which correspond to the distribution of the
Golgi apparatus in the hepatic parenchymal cell--
along the bile canaliculi, adjacent to the nuclei, andbetween the nuclei and bile canaliculi (Figs. 2 and
3). Reaction product was also present along the
sinusoidal borders of most of the cells. In Fig. 2
(top), specific reaction loci along the bile canaliculi
are especially prominent. The perinuclear andperisinusoidal deposits of reaction product are
also shown to advantage. Fig. 3 (top) is a low magnification
view which emphasizes the widespread
occurrence of the specific reaction. The distribution
of reaction product was the same whether the
Fab used was made from anti-VLDL or anti-LDL.
The same pattern and intensity of localization were
obtained when the indirect method was used (1).
Thus, the use of the direct method did not cause a
detectable loss in sensitivity. A generalized brown
staining of the cytoplasm was present in both experimental
and control sections and was, therefore,
interpreted to be nonspecific. Kupffer cells lining
the sinusoids of both experimental and control
sections contained reaction product in their cytoplasm
(Fig. 3, bottom), probably owing to the activity
of their endogenous peroxidase.