The amplified products of species-specific simplex PCR were visualized firstly on 2% agarose gel stained with Florosafe DNA stain (1st base Laboratories, Selangor, Malaysia) using a gel image documentation system (AlphaImager HP, California, USA) (data not given) and secondly on gel-mage and electrochromatograms of Bio-Rad Experion Automated Electrophoresis