The previous results were
coincidentally with an obvious accumulation of both flavonoids and total phenolic compounds in
cultures treated with SAE. The complementary between the previous results manifested the longevity
and viability of calli on modified MS medium. Antioxidant activity also confirmed the different
results. Successful suspension cultures were established using MS medium without vitamins and
phytohormones and supplied with 18% SAE. Growth parameters, total flavonoids, phenolic compounds
and antioxidant activity were highly increased compared with the untreated cultures.