Generally, to degrade cellulose, acid treatment with sulfate is carried out, and then the solution is neutralized and used for ethanol fermentation. However, the residues by neutralization generate environmental load. On the other hand, degradation by enzymes, such as endoglucanase (EC. 3.2.1.4), β-glucosidase (EC. 3.2.1.21) and others, proceeds under mild conditions and emits less residues. The fermentation of glucose to ethanol is performed by various microorganisms including yeast. To date, the direct production of ethanol from cellulosic materials has been under development; the laboratory strain of Saccharomyces cerevisiae displays cellulolytic enzymes on its cell surface and can simultaneously saccharify and ferment ( 2). However, the growth and ethanol productivity of laboratory yeasts are inferior to those of practical strains, including sake yeasts.