The membrane was washed and incubated in the streptavidinbiotinylated alkaline phosphatase complex for 1 h, followed by immersion in the color development solution until the purple bands became visible, usually within 10 min.
Themembrane was washed and incubated in the streptavidinbiotinylatedalkaline phosphatase complex for 1 h, followedby immersion in the color development solution until thepurple bands became visible, usually within 10 min.