The effect of producing and culturing Nile tilapia (Oreochromis niloticus) larvae in biofloc technology (BFT) based systems was investigated in terms of larval growth performance and robustness. Broodstock fish were maintained in a system with and a system without the application of biofloc technology in order to produce larvae. The survival of the larvae from BFT origin (90–98%) was higher than the survival of the larvae from control origin (67–75%). The growth performance of the larvae did not seem to be affected by the origin or the housing of the larvae, although the growth seemed to be more uniform when the larvae were housed in BFT water. Overall, the data show that the application of biofloc technology for tilapia brood fish maintenance and larval production can improve Nile tilapia fry quality and production performance.