Solid state fermentation
Experiments were carried out in 250 ml conical flasks, each of
which contained 100 g fresh sorghum stalks. Details of the cell
concentration, particle size and moisture content were described
in the following experiments. After inoculation, the contents were
mixed thoroughly and incubated at 37 °C. The solid samples
withdrawn from the fermentation medium were pressed using a
syringe to remove the liquid from the solids. This liquid was
centrifuged and then filtered to remove cells and other suspended
solids. The sugars and ethanol concentrations were determined in
this clear liquid phase