The re-suspended yeast cells were inoculated
into beet juice or synthetic medium to reach a yeast cell concentration
of 2.5 108 cell/ml. Fermentation flasks were incubated at
30 C, 150 rpm for 24 h. A 50 lL broth sample was withdrawn from
each flask at 2 h intervals. Each sample was diluted with de-ionized
water and centrifuged at 12,100g for 10 min before being
subject to sugar and ethanol assays. Fermentations with different
initial yeast inoculum (1 108 and 5 108 cell/mL of the SPSC01
yeast) were also investigated. The fermentation performance was
evaluated