HPLC conditions
Twenty micro liters of diluted coconut treacle
sample was injected onto a Supelco Gel C 610H
analytical column, (300mm x 7.8mm), Supelco,
USA at a temperature 30oC with a flow rate of 1.0
ml/min. Mobile phase was 0.1% H3PO4 (BDH, UK)
and detection was carried out on a Shimadzu RID-
10A, Shimadzu Corporation, Japan. All injections
were made in triplicate.