When initially attempting to isolate one of the many organisms found in the orange juice as Lactobacillus casei, the presence of gram positive rods and the negative results of the catalase test were the most important characteristics. Once we isolated an organism that fit the characteristics of Lactobacillus casei we preformed our confirmation tests to prove that the isolate was L. casei. Of the confirmation tests, the presence of clearing on the ADT CaCO2 plates was important, as clearing indicates the presence of Lactobacillus species. In addition, the acid production in the mannitol tubes, and the yellowing of the OF tube with oil, indicating the ability to ferment were also critical tests to confirm the presence of L. casei. The nutrient broth test, and the motility stab were further supports, but not absolutely critical to determine that the isolate was L. casei.