Prior to field sampling, each of the 54 split-plots (i.e., herbicided
and non-herbicided split) was divided into five ‘‘sub-plots’’ that
were anchored at the four corners and the center of each split-plot.
We used this method to ensure complete coverage of each splitplot.
In the field, sample points within each ‘‘sub-plot’’ were randomly
located interior to a three-tree outer buffer and between
two living loblolly pine trees. Occasionally samples were taken
within the buffer due to mortality within plots.
At each sample point, forest floor materials were collected
down to the mineral soil from a 0.25 0.25 m quadrat followed
by the extraction of a soil core. Soil cores (4.8 cm diam. 10 cm
deep) were collected with a split soil corer (AMS, Inc., American
Falls, ID, USA), and pooled by split-plot. All soil samples were kept
in a cooler with ice in the field and maintained at 4 C in the lab
until processed.
Prior to field sampling, each of the 54 split-plots (i.e., herbicidedand non-herbicided split) was divided into five ‘‘sub-plots’’ thatwere anchored at the four corners and the center of each split-plot.We used this method to ensure complete coverage of each splitplot.In the field, sample points within each ‘‘sub-plot’’ were randomlylocated interior to a three-tree outer buffer and betweentwo living loblolly pine trees. Occasionally samples were takenwithin the buffer due to mortality within plots.At each sample point, forest floor materials were collecteddown to the mineral soil from a 0.25 0.25 m quadrat followedby the extraction of a soil core. Soil cores (4.8 cm diam. 10 cmdeep) were collected with a split soil corer (AMS, Inc., AmericanFalls, ID, USA), and pooled by split-plot. All soil samples were keptin a cooler with ice in the field and maintained at 4 C in the labuntil processed.
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