Trait Measurements
Birds were weighed as a group on arrival and at 18 d of age at the termination of the studies. Feed intake was also recorded at the same time points for determination of feed conversion ratio (FCR; feed intake/weight gain). Mortality was recorded daily and feed conversion data corrected for BW of mortality. At the conclusion of the study, all chicks were killed by CO2 inhalation. The right tibia from 2 randomly sampled chicks per pen and toes from all chicks per pen were
removed and ashed to determine bone mineralization (Yan et al., 2005). Briefly, all the toes of all chicks were
dried for 24 h at 105°C, and the ash determined on pooled samples of toes from each pen. Tibia bones were cleaned of adhering tissue, fat was removed by extraction with ether and alcohol and dried, and ash content determined on pooled samples of all bones from each pen (AOAC International, 2005). The ash content was expressed as grams of ash per 100 g of bone dry weight.
Trait Measurements
Birds were weighed as a group on arrival and at 18 d of age at the termination of the studies. Feed intake was also recorded at the same time points for determination of feed conversion ratio (FCR; feed intake/weight gain). Mortality was recorded daily and feed conversion data corrected for BW of mortality. At the conclusion of the study, all chicks were killed by CO2 inhalation. The right tibia from 2 randomly sampled chicks per pen and toes from all chicks per pen were
removed and ashed to determine bone mineralization (Yan et al., 2005). Briefly, all the toes of all chicks were
dried for 24 h at 105°C, and the ash determined on pooled samples of toes from each pen. Tibia bones were cleaned of adhering tissue, fat was removed by extraction with ether and alcohol and dried, and ash content determined on pooled samples of all bones from each pen (AOAC International, 2005). The ash content was expressed as grams of ash per 100 g of bone dry weight.
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