and then evenly sprayed
on the surface of maize leaves. After 6 h, the maize leaves were
inoculated by evenly spraying with the conidiospores of B. maydis.
The seedlings were first cultivated in the dark overnight at 24 C
and 98% relative humidity and then cultivated in a growth chamber
with 16-h-light and 8-h-darkness cycle at 24 C and 85% relative
humidity (Mu, 2000). Each treatment, in triplicate, was arranged in
a completely randomized block with 3 30 plants. Spraying with
diluted LB medium and conidiospores of B. maydis served as
a negative control, and spraying with distilled water served as
control. After pathogen challenge, the disease was observed every
2 days. Disease index and protection effect by CHM1 were visually
rated by assessing the percentage of leaf surface covered by disease
spots.