Production of transgenic gentian plants
To suppress F3′5′H and 5/3′AT genes in gentian plants, a binary vector pSMABR-rolCpro-intF3′5′H:5/3′ATir ( Fig. 2A) was constructed. The chimeric RNAi construct was driven by a rolC promoter from A. rhizogenes, which induced successful gene silencing by RNAi in gentian flowers in our previous study ( Nakatsuka et al., 2008b).