3.1.3. Effect of initial pH 6alue on cellulase synthesis
To evaluate the effects of initial pH value in solid substrate on cellulase synthesis, the initial pH values were adjusted by the addition of lime slurry to 4.5, 5.0, 5.5 and 6.0, respectively. The corncob residue with an original pH value 2.8 was also used in solid state cellulase protein was harvested, the solid substrate with mycelia and spores was used for the next batch fermentation, but neither fresh substrate nor inoculum wasadded. This process was repeated several times (Table2).