3.2. Acid hydrolysis
The sugarcane leaf litter acid treatment of 0.8M H2SO4,
fermenting for 12 days produced more ethanol than any
other treatment combination up to day 12. Fermentation
for more than 12 days did not increase ethanol production
(Fig. 4).
For acid hydrolysis, the optimal concentration of H2SO4
was 0.8M H2SO4. Results for sugarcane leaf litter show
that fermenting for 12 days was the most eYcient acid
hydrolysis treatment for ethanol production, producing
335.67mg/L ethanol (Fig. 4). In acid hydrolyzed experiments
of waste cotton conducted by Yu and Zhang (2004),
0.2mol/L H2SO4 was the optimal acid treatment, producing
14.2 g/L of ethanol in 24 h.
After comparing alkaline H2O2 and H2SO4 acid treatments,
it was shown that acid hydrolysis produced the most
ethanol from the residue. More ethanol was produced from
sugarcane leaf litter when treated with 0.8M H2SO4 for 12
days compared to alkaline pretreated residue at 2% H2O2
(pH 13) 8 h fermented for 10 days (Fig. 5). This preliminary study showed that ethanol production from post-harvest
sugarcane residue is possible. Lignin prevents the degradation
of cellulose mainly by acting as a physical barrier
between the cellulolytic enzyme and its substrate. Consequently,
the rate and extent of enzymatic cellulose degradation
in lignocellulosic materials is inversely related to the
lignin content (Gould, 1984) with maximum cellulose degradation
occurring only after 50% or more of the lignin
has been removed. In this study, we achieved a signiWcant
removal lignin from the sugarcane residue, which resulted
in higher production of ethanol. Further research is needed
to optimize the conditions for maximum production of
ethanol from the sugarcane residue.
3.2. Acid hydrolysisThe sugarcane leaf litter acid treatment of 0.8M H2SO4,fermenting for 12 days produced more ethanol than anyother treatment combination up to day 12. Fermentationfor more than 12 days did not increase ethanol production(Fig. 4).For acid hydrolysis, the optimal concentration of H2SO4was 0.8M H2SO4. Results for sugarcane leaf litter showthat fermenting for 12 days was the most eYcient acidhydrolysis treatment for ethanol production, producing335.67mg/L ethanol (Fig. 4). In acid hydrolyzed experimentsof waste cotton conducted by Yu and Zhang (2004),0.2mol/L H2SO4 was the optimal acid treatment, producing14.2 g/L of ethanol in 24 h.After comparing alkaline H2O2 and H2SO4 acid treatments,it was shown that acid hydrolysis produced the mostethanol from the residue. More ethanol was produced fromsugarcane leaf litter when treated with 0.8M H2SO4 for 12days compared to alkaline pretreated residue at 2% H2O2(pH 13) 8 h fermented for 10 days (Fig. 5). This preliminary study showed that ethanol production from post-harvestsugarcane residue is possible. Lignin prevents the degradationof cellulose mainly by acting as a physical barrierbetween the cellulolytic enzyme and its substrate. Consequently,the rate and extent of enzymatic cellulose degradationin lignocellulosic materials is inversely related to thelignin content (Gould, 1984) with maximum cellulose degradationoccurring only after 50% or more of the ligninhas been removed. In this study, we achieved a signiWcantremoval lignin from the sugarcane residue, which resultedin higher production of ethanol. Further research is neededto optimize the conditions for maximum production ofethanol from the sugarcane residue.
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