In in vivo experiments, C57BL/6 mice were infected with H. pylori by intragastric
inoculation (1 108 CFU/mouse) 3 times, and orally treated twice a day for 14 days with WRPE-EtOH and
WRPE-BuOH. On a CLO kit assay, 200 mg/kg of WRPE-EtOH fully eliminated the bacteria from the gastric
mucosa, and the effect of 100 mg/kg of ethanol fraction was similar to pantoprazole (30 mg/kg), displaying
75% elimination. WRPE-BuOH was more effective, exhibiting 75% elimination at 20 mg/kg.