Protease showed satisfactoryactivity from pH 5.0 to 7.0, with the highest value at pH 5.0 (55.65U/mL). Different pH0values were reported for optimal activity ofproteases from Aspergillus tamari (pH 6.0 to 9.5) and Aspergillusawamori (pH 4.0 and 5.0). Based on these results, the next setof experiments was performed at pH0= 5.0