A 500 mL aliquot of human plasma was diluted with 2 mL DW in
a 10 mL centrifuge tube. An aliquot of 500 mL Triton X-114 5% w/v
was added and homogenized using a vortex stirrer. The resulting
cloudy solution was kept at 65 C for 20 min for promoting the
condensation of surfactant micelles. Then it was centrifuged at
3000 g for 5 min for separation of the surfactant-rich phase. After
discarding the aqueous supernatant, 400 mL ACN were added to the