4. Conclusion
In this work, a novel method was developed for high throughput
determination of antioxidant based on a DPPH dry reagent array.
The method involved coating the surface of a 96 microplate
with DPPH, previously dissolved in methanol, by drying it under
nitrogen. The DPPH dry reagent array was applied successfully to
the determination of antioxidant activity in real samples and performed
as well as the fresh solution DPPH assay.
The proposed
method offers some advantages compared to the classical solution-
based assays. The advantages included but were not limited
to rapid, sensitive, cost-effective and convenient, used less reagents
and could be stored for up to 4 months.
Acknowledgement
This research was supported by Universiti Kebangsaan Malaysia
under grant No. UKM-OUP-NBT-29-139/2011.
4. ConclusionIn this work, a novel method was developed for high throughputdetermination of antioxidant based on a DPPH dry reagent array.The method involved coating the surface of a 96 microplatewith DPPH, previously dissolved in methanol, by drying it undernitrogen. The DPPH dry reagent array was applied successfully tothe determination of antioxidant activity in real samples and performedas well as the fresh solution DPPH assay. The proposedmethod offers some advantages compared to the classical solution-based assays. The advantages included but were not limitedto rapid, sensitive, cost-effective and convenient, used less reagentsand could be stored for up to 4 months.AcknowledgementThis research was supported by Universiti Kebangsaan Malaysiaunder grant No. UKM-OUP-NBT-29-139/2011.
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