The stability of D3 encapsulated in the blg/Lyso microspheres at
4 C was determined over a 5 week period. The initial concentration
of encapsulated D3 was determined after 24 h, as described
in Section 2.3. Samples corresponding to weeks 1–5 were stored
at 4 C. Each week, a sample was withdrawn for determination of
the amount of D3 remaining in solution for each condition. Solutions
of D3 diluted in MilliQ water were prepared and analysed
under the same HPLC conditions.