2.1. Chemicals
Inhibition studies were conducted using potassium-activated
ALDH enzyme from baker‘s yeast (Saccharomyces cerevisiae) in
lyophilised powder form (Sigma–Aldrich, St. Louis, Missouri,
USA) with b-NAD+ (Oriental Yeast Corporation, Osaka, Japan) as
cofactor. Dichloromethane, pentane, and authentic diethyl disul-
fide were procured from Wako Pure Chemical Industries, Ltd.
(Osaka City, Japan). All chemicals and solvents used were of analytical
grade and purity.