The significantly higher growth for all strains along with P09 were recorded at 25 °C
which is parallel to the result of Manivannan etal. (2012) also study the three R. leguminosarum strains isolated from L. japonicus and L. pratensis for low temperature tolerance, R. leguminosarum bv. viciae ATCC 10004 and commercial strain 175P1 were used as reference strains for cold sensitive and poorly adaptive strains.