it is assumed that the number of endosymbiont bacteria is frequently
reduced until apparent total disappearance from gill tissue of musselsmaintained for at least 2–3 weeks in plain sea water and exposed to
atmospheric pressure. Nonetheless, there is evidence that some
endosymbiont bacteria remain in gill tissues after months in sea
water aquaria supplemented with methane and sulfide and subjected
to atmospheric pressure conditions (Dando et al. and Colaço et al.
unpublished results).
Themaintenance of live vent mussels in our laboratory has been a key
factor in gaining insights into the physiology of vent mussels. It has
prompted us to investigate cellular and molecular mechanisms of
adaptation in B. azoricus during long term post-capture aquaria conditions
(Kadar et al., 2005;Bettencourt et al., 2007). The presence of gill-associated
mucopolysaccharides, changes in hemocyte glycoproteins, endosymbiont
content of gill tissues and phagocytosis were selected as biological
indicators of B. azoricus physiological state under long term aquarium
conditions. The decrease of dense droplets of mucus-like granules at the
ventral end of lamellae of gill filaments transverse sections was followed
over a period of 6 months. In addition, the immunocompetence of
Bathymodiolus hemocytes was assessed by phagocytosis after long term
maintenance in aquarium conditions at atmospheric pressure.
it is assumed that the number of endosymbiont bacteria is frequently
reduced until apparent total disappearance from gill tissue of musselsmaintained for at least 2–3 weeks in plain sea water and exposed to
atmospheric pressure. Nonetheless, there is evidence that some
endosymbiont bacteria remain in gill tissues after months in sea
water aquaria supplemented with methane and sulfide and subjected
to atmospheric pressure conditions (Dando et al. and Colaço et al.
unpublished results).
Themaintenance of live vent mussels in our laboratory has been a key
factor in gaining insights into the physiology of vent mussels. It has
prompted us to investigate cellular and molecular mechanisms of
adaptation in B. azoricus during long term post-capture aquaria conditions
(Kadar et al., 2005;Bettencourt et al., 2007). The presence of gill-associated
mucopolysaccharides, changes in hemocyte glycoproteins, endosymbiont
content of gill tissues and phagocytosis were selected as biological
indicators of B. azoricus physiological state under long term aquarium
conditions. The decrease of dense droplets of mucus-like granules at the
ventral end of lamellae of gill filaments transverse sections was followed
over a period of 6 months. In addition, the immunocompetence of
Bathymodiolus hemocytes was assessed by phagocytosis after long term
maintenance in aquarium conditions at atmospheric pressure.
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