2.2. Cultures conditions
The ITE, that was poor in nitrogen (N) and phosphorus (P) elements
and their species such as NH4
þ or PO4
compounds, was
considered such a carbon © source for the growth of the selected
fungus. However, the urea (CH4N2O) and the KH2PO4 nutrients
were used as sources of N and P. For the optimization of medium
composition they were used with concentrations of 0.15 g/L and
0.5 g/L, respectively. In addition, the pH was adjusted to 5.5. These
variables were chosen on the basis of a preliminary trial about the
optimization of the medium composition for filamentous fungi
culture (data not shown). The corrected textile wastewater was
autoclaved and inoculated with 1.44 105 spores/mL. Cultures were
incubated at 28 C under static and stirred conditions (150 rpm) for
10 days. During the cultural run, a sample from the broth medium
was filtered to remove the fungal mycelium, and then centrifuged
at 3000 rpm for 15 min. The resulting clear solution was used for
enzyme activities assays